Phenetic and Molecular Diversity of Nitrogen Fixating Plant Growth Promoting Azotobacter Isolated from Semiarid Regions of India

In the present study, 24 Azotobacter strains were isolated from soils of different areas southern Rajasthan and characterized at biochemical, functional, molecular levels. The gram negative cyst forming when viewed under microscope. These also screened for their plant growth promoting activities ability these isolates to survive abiotic stress conditions viz. salt, pH, temperature, drought stress. All showed IAA, siderophore, HCN, ammonia production, whereas seven phosphate solubilization. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed significant diversity among dendrogram obtained differentiated twenty-four into two major clusters a similarity coefficient 0.64. Qualitative quantitative N2 fixation abilities detrained, amounts acetylene reduced by in range 1.31 846.56 nmol C2H4 mg protein−1 h−1. showing high nitrogen with multiple PGP selected further pot studies, significantly increased various parameters maize plantlets. Furthermore, best subjected 16S rRNA sequencing confirmed identities as sp. indigenous could be used commercial production.